Transcriptome Analysis Reveals the Effect of PdhR in Plesiomonas shigelloides
نویسندگان
چکیده
The pyruvate dehydrogenase complex regulator (PdhR) was originally identified as a repressor of the pdhR-aceEF-lpd operon, which encodes (PDHc) and PdhR itself. According to previous reports, plays regulatory role in physiological metabolic pathways bacteria. At present, function Plesiomonas shigelloides is still poorly understood. In this study, RNA sequencing (RNA-Seq) wild-type strain ΔpdhR mutant strains performed for comparison identify PdhR-controlled pathways, revealing that regulates ~7.38% P. transcriptome. We found deletion pdhR resulted downregulation practically all polar lateral flagella genes shigelloides; meanwhile, motility assay transmission electron microscopy (TEM) confirmed non-motile lacked flagella. Moreover, results RNA-seq quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) showed positively regulated expression T3SS cluster, significantly reduced ability infect Caco-2 cells compared with WT. Consistent research, pyruvate-sensing directly binds its promoter inhibits operon expression. addition, we two additional downstream genes, metR nuoA, are negatively by PdhR. Furthermore, also demonstrated ArcA being located upstream lpdA regulating their Overall, revealed pathway PdhR, will allow more in-depth investigation into pathogenicity well network.
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اندیشمندان و صاحب نظران علوم اجتماعی بر این باورند که مرحله تازه ای در تاریخ جوامع بشری اغاز شده است. ویژگیهای این جامعه نو را می توان پدیده هایی از جمله اقتصاد اطلاعاتی جهانی ، هندسه متغیر شبکه ای، فرهنگ مجاز واقعی ، توسعه حیرت انگیز فناوری های دیجیتال، خدمات پیوسته و نیز فشردگی زمان و مکان برشمرد. از سوی دیگر قدرت به عنوان موضوع اصلی علم سیاست جایگاه مهمی در روابط انسانی دارد، قدرت و بازتولید...
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2023
ISSN: ['1661-6596', '1422-0067']
DOI: https://doi.org/10.3390/ijms241914473